Who Makes the final Decision on ISAC Congress-Purdue Cytometry Mail List if ADVERTISING is Allowed or Not?
From: J. Paul Robinson j…@flowcyt.cyto.purdue.edu
Date: Mon Mar 31 2008 - 01:18:05 EDT
From J. Paul Robinson - Moderator
Robert is right - there is too much politics and not enough
science…
What is happening here, is that there are too many cooks.
Let me make it very clear that we work hard to keep this list clean.
It does not always work.
When we identify a failure,
we usually respond
to
the person concerned
and
don’t waste all your time.
We frequently note in our posts,
that
advertising is not allowed.
This list was developed from 2 or 3 individuals who actually had email
in 1990,
to 3000 over the past 19 years.
t did not happen by chance,
nor was it overnight.
It was developed with a lot of cost ($$$), a lot of time,
and what was a pretty darned good idea when it started.
We don’t tolerate people who try to damage the list.
Now that it’s highly successful,
there are a number of individuals
that
are trying to either circumvent the list,
use it for their own purposes,
or simply sideline it.
There are even some proposing
that
they should be able to manipulate the list
and i
ts contents
in any forum
for any purpose.
I am really shocked at this rather callous approach
to a scientific discussion board.
I am not making public those individuals
or
their companies,
but if I am pushed,
I will identify them publicly.
If
I do so, and they create havoc,
it could shut the list down -
or end up
in
a nasty legal battle.
I don’t suppose that would be popular?
So,
it seems to me,
that we need to get back to basics and focus on
the
reason this list has been so successful
(and why you all want to use it for advertising -
or even why those who what to hijack it…)
- it
does a good if not great job overall.
thanks for your support -
all 3000 of you …
well most of you!
Clearly we will provide a mechanism
for companies to provide a means
for
c
ommunication….i
t’s on our list…
Maybe I am getting too old for all this abuse….!
regards
Paul Robinson
Purdue University
From: Adam Treister
(a…@treestar.com)
Date: Mon Apr 22 2002 - 00:15:07 EST
* Reply: Mario Roederer: Job Opening — Immediate — Vaccine
______________________________________…
Only two more weeks until ISAC,
that biennial bacchanalia of flower
power
and fun!
So I hope you’ll excuse a bunch of blatantly commercial
announcements to the list,
but endulge me this one time and read on.
At the show we’ll be releasing FlowJo Version 4. We’ve got new
platforms
for overlaying and clustering, we’ve made it work better across the
Internet, added all sorts of new
For those who haven’t found it yet, we’ve unveiled a ******** new
FlowJo
website.
Flowjo.com
is chuck full of new content, functionality
and
spunk.
Automated price quotes,
online ordering,
FAQ that will guide you to
new
depths of understanding,
and none of that awful yellow on black text.
The
search engine even works.
No ads cookie-free
Check it out.
************************************
Specifically,
you should check our pricing.
Prices are going up on May 10.
************************************
It has been a number of years since we’ve changed our prices
and
with
the
development of the OSX and PC versions,
it¹s time for a leap.
I
guess
there’s no such thing as a free launch.
Anyway,
this may be a great
time to
buy that FlowJo ten pack you’ve been thinking about.
Licenses
purchased
before
May 10 are entitled to a year of free upgrades,
including the
4.0
release.
Unleash the flower power!
Adam
–
Adam Treister
Tree Star,
Recent FlowJo announcement
From: Steve Kelley
(SKEL…@flowcyt.cyto.purdue.edu)
Date: Wed Dec 30 1998 - 06:13:04 EST
Next message: Steve
Kelley: Possible minor disruption
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I know that many people on the mailing list are adamantly opposed to
anything that even looks a little commercial, and to try to forestall
any possible
complaints about the FlowJo announcement
, I’ll explain what
I’d like to see. In my opinion, announcements about new products, and
product updates are completely appropriate as long as they aren’t
abused.
I’ve never wanted to specifically encourage that, because I really
don’t want to be put into a position of having to decide whether a
message is an
announcement
or an
advertisement
. The companies involved in our cytometry community have always been
extremely good ‘citizens’ as far as I can tell.
We have representatives of many companies on the list, and they have
always had the power to make my life miserable, and the list no more
than junk mail.
Instead,
they
have helped build this mailing list
into
one of the most useful around, through
their contributions
and
responses.
I’m not going to try to set specific rules about what
people can say about their own products,
and when they can say it.
I’ll just ask that everyone continue to show restraint;
that the
commercial representatives
ask themselves before they submit an
announcement
whether they’d mind seeing every other company
in the
business sending the same message they are about to,
and
that the
non- commercial
(and anti-commercial)
people accept discreet announcements
as simple information,
and continue the sometimes brisk discussions
about problems and benefits of particular products,
alongside the
purely scientific
(and occasionally purely entertaining)
conversation.
Steve Steve Kelley
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From: Ryan Farmer far…@treestar.com *********** WEBMASTER
Date: Mon Apr 14 2008 - 19:05:51 EDT
There is another site of interest along these lines.
Mycyte.org
is well established and has a variety of online texts, articles and
other links (protocols, standards, etc.). Users can expand the
variety
and
amount of information as well.
It is located at http://www.mycyte.org.
Ryan Farmer
TreeStar Inc.
340 A St. Suite 301
Ashland, OR. 97520
far…@treestar.com
(541) 201-0022 ext. 172
www.treestar.com
www.flowjo.com
www.flowdx.com
www.mycyte.org
Received on Tue Apr 15 13:58:00 2008
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Flow Cytometry on the Web
Software -New releases
From: Benjamin C. Hunsberger (75450.167@CompuServe.COM)
Date: Fri Oct 29 1993 - 10:44:42 EST
·Next message: Brian Hall: For Sale: 1989 Coulter Profile II
·Previous message: jm5@maties.sun.ac.za: SOFTWARE PROGRAMS FOR FLOW CYTOMETRY
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Verity Software House, Inc. has just released WinList 2.0 and ModFit 5.2.
All registered users will be receiving update notices by mail. We have new
demo disks for these products. Any one wishing information and/or a free
demo may leave a message on Internet or FAX us at 207-729-5443. Please
include your FAX number.
We have also produced two video tutorials (VHS format) for WinList. Tape 1
is at the beginner - intermediate level, Tape 2 is at the intermediate -
advanced level. Each tape is 2 hr’s long and has a price of $75.00. New
orders for WinList received by Jan. 1, 1993 will receive both tapes free.
Verity Software House, Inc. is now the exclusive distributor for TechTeam’s
Truploid software. Truploid allows you to quantitatively evaluate your DNA
listmode files for drift, bulges and standard deviation then if desired
remove part of the acquisition and create a new file. Again you may leave
a message on Internet or FAX us for more details.
Don Herbert
Verity Software House, Inc.
10 New Lewiston Road
Topsham, ME 04086
Phone 207-729-6767
FAX 207-729-5443
·Next message: Brian Hall: For Sale: 1989 Coulter Profile II
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Re: Thanks for the suggestions - was rendering in 3D
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From: Adam Treister …@treestar.com
Date: Thu May 18 2006 - 20:20:18 EDT
On May 12, 2006, at 10:02 AM, Bushnell, Timothy wrote:
Thanks to everyone who suggested possible software to view data in
3D. I’ll be trying several different platforms to see which works
best for our applications.
The suggested platforms include (in alphabetical order):
Coulters CXP software for the FC500
Rflowcyt
Weasel: http://www.wehi.edu.au/cytometry/WEASELv2.html
Winlist 3d from Verity House Software (www.vsh.com)
Regards
TimTim,
With all due respect to these solutions,
you shouldn’t think that
Mario could sleep at night
if anyone could perform high dimensional analysis better than FlowJo.
Disclaimer:
Yes,
I live off FlowJo sales,
and that’s a blatantly commercial statement,
but
it gets technical from here on.]
We’ve played with spatial 3D plots a lot, both our own prototypes
and
others,
and they just don’t do a very good job of discriminating populations.
And its impossible to coherently describe the populations you can see.
I like the slice-and-dice approach that
you get by making a flipbook on X vs. Y in slices along the Z axis
(it shows up as a Quicktime movie),
but that too is very difficult
to
use in a way that’s better than two 2D graphs connected by a gate.
If you really want to increase your dimensionality,
we’re just
adding
a new Polyvariate Plot to the Mac version of FlowJo
for next week’s ISAC.
The idea was taken from RFlowCyt.
We’ve added interface
refinements to make it more interactive,
but like any good R tool,
it’ll astound and confuse you.
http://www.flowjo.com/v8/html/polyvarplot.html
The Polyvariate Plot can model transformations in any number of
dimensions.
So it will produce a 3D plot, or as many dimensions as
you want (Shown below in 5D).
And it projects these transformations
onto a graph window, so you can gate on them.
We’re still trying to figure out the applications for this
visualization,
but if you’re looking for another dimension as a way
to differentiate populations,
we think this is potentially much more
powerful than conventional spatial projections.
This is explained
in the poster P178
at
ISAC next week,
or at the web page above.
Be forewarned:
This is the opposite end of the
sizzle/steak
spectrum.
Most people use 3D graphs to make their PowerPoints look
spiffy.
These graphs are absolutely impossible to explain in a
presentation.
Adam
———————–
A 3D plot:

A 5D plot:

Received on Fri May 19 17:58:00 2006
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rendering in 3D
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From: Adam Treister a…@treestar.com
Date: Sun Jan 04 2004 - 13:48:24 EST
David,
We’ve tried on a couple of occasions to add a spatial 3D module to
FlowJo, and it has never turned out well enough to make it into a
release.
We’ve found that using time as the third dimension, as in
our
data movies, or using several 2D graphs, as in our multi-graph
overlay to be more practical solutions.
If you want smoothing or density coloring, that requires binning the
data. Even working at low resolution, you’re looking at 256 times as
much time and memory to take the plot into an additional dimension.
You might get a tenfold performance increase with the G5 (which I think is quite optimistic,
because the G5 adds fast floating point processing,
but binning is a integer operation), but even with that
that, adjusting a gate goes from taking perhaps a second to almost a
half minute.
That would make using
FlowJo feel like using CellQuest
(just kidding 
At the full resolution of DiVa files, you’re
looking
at another thousand fold increase over the 2D version, or a billion
times (1000 ^ 3) as long as we take to do it now.
So, as best I
can
figure it,
we can only support 3D at the cost
of losing interactivity
with the data
(ie, we can make the views, but changing gates or
parameters won’t immediately change the 3D visualization).
It would be tough to have contours in 3D as each layer would obscure
the ones inside it.
Contours would have to have varying opacity,
which
not only increases the computational time and complexity,
but would
make it hard to differentiate populations. And the user interface
for
gating in space would be a real challenge.
You could chop thru space
with planes, but that’s 1D gating, which doesn’t give you more
capability to define populations than you have now.
So we’d have to
invent polyhedral gating.
If all you want to do is look at already-gated populations in 3D,
there
are options that exist.
Expo32 has this feature, if you can figure
out
how to use
Beckman Coulter
(actually, ACS wrote it)
software to view BD files.
You can use existing commercial programs
(Aabel JMP are nice Mac 3D applications, and I’m sure there are others),
or write it yourself in OpenGL.
I think OpenGL would be a better choice than C+ +.
OpenGL is a higher level graphics language, and knows how to access
the specialized accelerators in the graphics cards, which are actually
faster for this stuff than the G5.
That’s how the dungeon games do
the 3D shading and rendering.
I’ll be happy to donate a considerable amount of code that I’ve done in this effort
(most was
taken
from
an old freeware program
called
Rotator,
which I no longer could find with Google
but I have
somewhere
in my archives),
but we decided this was pretty much a dead end.
Rotator was in C, and quite unreadable. For the investment this task
would take, I think it’d be better to start over in OpenGL. It’s cross-platform too, which is important, as you’ll find you want to
port
it to a Cray.
I’d still think you want to use FlowJo to read the DiVa files,
compensate them, transform them, gate them, and then export desired
subpopulations to the 3D viewer.
If it were any other instrument,
you
could probably read the files yourself,
with R or our free Java
libraries,
but the DiVa files are a unique format, and almost always
require compensation
and transformation to a lin/log scale, so
there’s
a lot of work before you even get to viewing them.
I’ve promised you 3D graphs in FlowJo in the past, and I’ve done my
best to deliver them, but the results have been pretty
disappointing.
And the benefit of them has never been demonstrated. If you can show
us how 3D views provide more interpretable data than our current
compromise solutions, that would help. If you want to pick a
data
file, we’ll make you get a spinning, stereoscopic, 3D view of it. If
we find that other scientists are able to make conclusions about the
data better than they can from our existing visualizations, that will
go a long way towards bumping it up on the FlowJo future feature
list.
I hope that helps.
Adam
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17:35:29 EST
From: J. Paul Robinson
Reply To: j…@flowcyt.cyto.purdue.edu
Sent: Friday, August 20, 2004 2:25 PM
To: Cytometry Mailing List
Subject: Re: mr on Apple web site
ummm….Mario says..
In life sciences - particularly in research life sciences -
probably 50 to 70% of research laboratories used
Macs….while I have a passionate dislike for Windows……is
this really true ??? or is the key word there used?? (Ok…I
have put on my helmet and armor….waiting…)
paul
For all of the mr groupies out there in cytometry cyberspace. Don’t wet
your
pocket protectors over this.
Honestly though, well deserved praise for Mario the Tree Star group:
http://www.apple.com/science/profiles/roederer
**********************************************************************************************
Re: exporting raw data from gated events
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From: user facs_copy facs_copy@wehi.EDU.AU
Date: Sat Jul 31 2004 - 03:18:32 EST
Chris Halweg asks via Janet Dow:
My question is how can I export the raw data within a gated region?
If you want to export list mode text, WEASEL can do that for any pair of
parameters from a dot plot. The gate can be a logical expression
involving several regions, rectangular or polygonal on any combinations of
parameter pairs. However it will not save gated data in FCS mode. See
http://www.wehi.edu.au/cytometry/WEASELv2.html for download. Go to
WEASEL’s Help section for further instructions.
Frank Battye.
| | The Cytometry Lab. facs_copy t wehi d0t edu d0t au
\__/ The Walter Eliza Hall Institute
——!! 1G Royal Parade, Parkville
/!!\ Victoria 3050, Australia
o !! \ ph: 61_3_9345 2541, fax: 61_3_9347 0852
Received on Tue Aug 3 12:58:00 2004
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Re: exporting raw data from gated events
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From: janet dow jldow@unity.ncsu.edu
Date: Mon Aug 02 2004 - 12:47:04 EST
Thank you to everyone who sent suggestions-my client has many options
now. He was very surprised and impressed with the speed and
abundance of replies. Too bad he is only a graduate student.
here is a listing of the programs suggested:
1) FCS Express (http://www.denovosoftware.com/) will do it.
2) WinList 5.0 (for PC or for Mac) from Verity Software House can export a
gated listmode file in FCS format or in ASCII text format. The gate can be
a simple gate or a Boolean gate expression, and some or all of the
parameters can be selected for export, including compensated parameters.
For more information, visit our web site, www.vsh.com, or contact me
directly.
3) Don’t know about the other software you mention but FlowJo will
certainly do what you want.
http://www.flowjo.com/v6/html/export.html
4) here is a possible solution with WinMDI. It is a little more
tricky than other software (like WinLIST or FCS Express) but it is
free and use WinMDI.
Draw your region in your dor plot, then gate this region only on your
dot plot in order to display only the cells you want. Now go to
File/Save as/ and save your file still in FCS format but with a
different name. WinMDI will save only the particles displayed on your
plot in the gate.
Now, use LLDATA (link :
http://www.cyto.purdue.edu/flowcyt/software/ldatapp.htm) which is a
freeware we developped here in Purdue to convert FCS file into a text
file (.txt). Because LLDATA can convert a bunch of files, the most
time consuming part is the gating and saving in WinMDI.
I hope it helps.
5) You can get the gated data directly from CellQuest by using the Save as
FCS file under the File menu, after creating a gated 2-d plot.
You would then use WinMDI to get the list in Excel format though). Use
the
Save as again, but choose Tabbed Text and save all parameters.
The data will be in channel values, so you’ll need to transform log data
(eg 10^(channel/256))
I haven’t posted the senders names but can give them if someone wants
to communicate directly about a particular question.
Thanks again
Janet
–
Janet Dow
Laboratory Research Specialist and Manager
Flow Cytometry and Cell Sorting Facility
North Carolina State College of Veterinary Medicine
Room C-309
Raleigh, NC 27606
(919)513-6443
Received on Tue Aug 3 13:58:00 2004
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Re: exporting raw data from gated events
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Re: exporting raw data from gated events
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From: Jennifer Wilshire jennifer@flowjo.com
Date: Tue Aug 03 2004 - 13:31:02 EST
Dear Chris,
You can use FlowJo to export flow data into a spreadsheet. You have choices
of exporting gated or not, compensated parameters only or all, and you can
choose specific parameters to export. To export, simply click on the name of
the gated subset in the Workspace window and choose the Export option from
the Workspace menu.
Just out of curiosity, what kind of analysis are you trying to do that isn’t
handled by any of the software products out there?
Please let me know if you have any questions.
Thanks,
Jennifer
–
Jennifer Wilshire, PhD
Application Scientist
Tree Star, Inc.
www.flowjo.com
617-784-4073
flowjo@treestar.com
Re: FloJo versus Paint-A-Gate
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From: Maciej Simm simm@cd4cd8.com
Date: Thu May 13 2004 - 16:29:19 EST
JoAnn,
The first step in identifying rare populations is finding a good
antibody that will be bright enough to separate your cells of interest
from the rest of your sample. If you have a fluorescently conjugated
antibody that will specifically detect B cell clones or residual
leukemias, FlowJo will help you visualize (and identify) these
populations when you view the data as a contour plot with outliers.
You can obtain the software for a 30 day evaluation from
http://www.flowjo.com/flowjo.html for the Macintosh Platform (OS9.2, OSX
10.2.8 and 10.3.3+)
or
http://www.flowjo.com/win/ for the Windows
and you will need a serial number from
http://www.flowjo.com/try.html
Maciej Simm
Tree Star Inc.
Re: Creating a database of FCS files
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From: Marty Bigos mbigos@gladstone.ucsf.edu
Date: Tue Apr 27 2004 - 11:05:04 EST
Hi Adrian -
The need of a management system for flow cytometry (as well as
microscope image) data has been apparent for quite some time. Not
only would it help in the research aspects you mentioned, it could
also provide many other functions, such as secured sharing of data,
easy availability from any networked site, etc.
For several years I have been fortunate to be involved with Bill Hyun
of UCSF through an SBIR with a local software company in SF (Biotrue,
Inc.) to develop such a system. We are in beta test now.
The product, (which doesn’t have a formal name yet, but is informally
called RDMS - Research Data Management System), will be announced at
the ISAC meeting at the end of May.
Biotrue
will have a booth there
to give demos of the product,
and I will be giving a workshop talk on
some of the ideas behind its design
.
*****************************************
I do have a (small) financial interest in Biotrue,
******************************************
but even if I
didn’t
I would recommend that you look at the product as a possible
solution to your needs.
You can contact Jenny Liu (JLiu@Biotrue.Net)
for more information.
Marty
Marty Bigos
Director, Flow Core
Gladstone Institute of Virology and Immunology
Building 3 SFGH Rm 509
415-695-3832
Hi all,
Some of the users here have raised the desirability of having a
database of all the FCS headers from all their data files. They
could then, for example, search for all the files/experiments in
which they used a particular stain etc.
Is anybody doing this? Would this be something that other people
would find useful?
I would love to set something up but I don’t have the requisite
skills or time at the moment.
As a temporary measure I suggested they export the FCS header info
from FlowJo using using a table and then compile them all in another
program like excel. This works for a few experiments but it needs to
be automated (and easy) if it is going to be generally applicable.
Any comments or suggestions?
Adrian Smith
Centenary Institute of Cancer Medicine and Cell Biology
Sydney, Australia
–
Received on Tue Apr 27 13:58:00 2004
RE: ISAC - MultiColor Flow Cytometry
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From: Marty Bigos mbigos@gladstone.ucsf.edu
Date: Tue Apr 27 2004 - 19:21:42 EST
Just to give credit where it is due:
The presentations at this workshop were (in order of appearance):
Marty Bigos - Gladstone Institutes - Basic Concepts for Compensation
Mario Roederer - NIH - The Hard, the Bad, and the Ugly
Nicole Baumgarth - UC Davis - The How-to Guide to Compensation for
Multicolor Cytometry
Panel - the above 3 joined by Bill Hyun (UCSF) and David Parks (Stanford)
The workshop was organized by Treestar, Inc. and the Gladstone Flow
Core. Many companies contributed to making it happen (in alphabetical
order):
Amnis, BD Biosciences, Beckman-Coulter, Caltag, DakoCytomation,
DeNovo Software, FloCyte Associates, Miltenyi, and Southern BioTech.
Paul Robinson and Bartek Rajwa did the video post-production.
Marty
The question has been asked about videoing ISAC
workshops and tutorials. On CD8 which will be coming out
at ISAC, we have a bonus. CD8 is a 2 cd set and the
second CD is an outstanding tutorial that Mario and Marty
Bigos and others put on in San Francisco last year. Its really
good!
We would like to do this for selected tutorials even at ISAC
and we are developing a proposal for ISAC as we speak. It
would help to know if this was something of interest.
Regards
paul robinson
Purdue
On 26 Apr 2004 at 11:03, Kroeger, Jodi wrote:
What are the chances that this tutorial, (and others of high
interest) could be
video taped and broadcasted over the internet for those
unfortunate souls unable
to attend? At least to those of us who are ISAC members?
—–Original Message—–
From: Mario Roederer [mailto:roederer@drmr.com]
Sent: Thursday, April 22, 2004 11:48 PM
To: cyto-inbox
Subject: ISAC - MultiColor Flow Cytometry
For the second straight ISAC (officially making this a tradition),
I’ll be running a Saturday tutorial on Multicolor Flow Cytometry.
This time, instead of being at the unhappy hour of 8 in the morning,
it will be at a much more civilized early afternoon time.
This tutorial (aptly numbered: Tutorial X) will be much more
pragmatic in nature than previous. We will have three talks that
will center on how to plan and implement a multicolor
immunophenotyping experiment, including instrument setup and
validation (Steve Perfetto), multicolor panel design and optimization
(myself),
and bridging the gap to the clinic (Brent Wood).
Assuming
that most of us are not in a full post-prandial siesta mode, this
should be a highly interactive session: please come with your
questions, data, artefacts, and complaints in hand!
If you are jumping on the 7, 10, or 15-color bandwagon, then this
tutorial is for you! If you’ve never done more than 2-color
staining, then enjoy the wonderful city of Montpellier instead 
If you have any suggestions for specific topics, or any general
questions, please send them to me in advance and we will try to
address them.
Regards,
mr
######################################################################
This transmission may be confidential or protected from disclosure and
is only for review and use by the intended recipient. Access by
anyone else is unauthorized. Any unauthorized reader is hereby
notified that any review, use, dissemination, disclosure or copying of
this information, or any act or omission taken in reliance on it, is
prohibited and may be unlawful. If you received this transmission in
error, please notify the sender immediately. Thank you.
######################################################################
J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
Have you seen our new HCS webpage?
http://www.cyto.purdue.edu/hcs
Received on Wed Apr 28 16:56:53 2004
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Previous message: Cris Bare: Re: RFI’s on longitudinal data (question restated)
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From: J. Paul Robinson jpr@flowcyt.cyto.purdue.edu
Date: Fri Jan 23 2004 - 02:48:05 EST
Colleagues
Some info related to current events:
Cytometry email: We want to keep spam from you
- but we
cannot stop spammers stealing addresses from our website
- my own address or address such as CDrominfo@flowcyt
for example, has been used by spammers - naturally, we
are not sending these out -
just to let you know that the
spammers are really doing nasty things. Beware!
CD-ROM 8: Cytometry cdrom is underway - we need your
contribution and support - there is not much time -
http://tinyurl.com/37v5a
183 have taken the core managers survey - I suspect this
the most comprehensive evaluation of core facilities ever
taken. There is a brief report at the end of this message -
the survey is still open.
183 have taken the core managers survey - I suspect this
the most comprehensive evaluation of core facilities ever
taken. There is a brief report at the end of this message -
the survey is still open. Please continue into the detail
survey if you do the first part please. If you don’t give us
your contact details…we can’t follow up.
592 people have taken the Future of Cytometry survey.
Again, this is the largest survey ever taken in cytometry. If
the results of this survey do not impact us, nothing can.
http://tinyurl.com/yq17 if you have not done it.
Core managers survey http://tinyurl.com/2r5p5
————————————————————–
A brief report on the Core managers Survey so far:
183: total respondent labs so far
120: provided full contact details ( the follow-up group)
110: are members of ISAC (59 did not tick any society)
163: do flow cytometry
145: do sorting
84: do fluorescence microscopy
40: do confocal
74: labs have ALL inst. on service contracts 53 have SOME
53: labs fix instruments themselves
590: total cytometers (34 labs did not report numbers)
56: confocal microscope (we have company breakdowns)
9: Multiphoton scopes
59: labs are Cancer Center associated, 92 are not, 32 did
not supply a response
130: core managers said they would join a new group to
address their concerns, 25 would not join and 28 did not
answer the question.
—————————————————————–
Full details in a few weeks.
Best wishes
J. Paul Robinson
Purdue University
J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
Received on Fri Jan 23 15:18:00 2004
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ISAC Montpellier CyberCafe
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From: Adam Treister a…@treestar.com
Date: Tue May 11 2004 - 17:28:22 EST
Dear ISAC Montpellier attendees:
We once will again be putting on an Internet cafe for your
gastronomical and connectivity pleasure at ISAC.
This year, we’ll be rechristening the establishment. Its has been
renamed the Fluor-de-lis. See our logo, below. We’ve adopted the
twelfth century crest of King Louis VII, but we’ve added more colors,
and installed a two-way sort. We hope to provide an oasis for you to
rest your feet and mind, check in with home, and enjoy an atmosphere
of
old world provincial charm, combined with blindingly fast packet
throughput.
Since every time we do this, it gets bigger and more elaborate (on a
log scale), we’ve invited a couple of new companies in to bring you
more space, more computers,
more food and drink.
We’re pleased to
share the sponsorship of the Fluor-de-lis with two young companies,
Biotrue (www.biotrue.net)
Cytopeia (www.cytopeia.com).
Both are introducing exciting new products for cytometry.
We think
you ought to know about them,
and they’ve agreed to
donate their
boothspace to be your connection to the outside world.
ISAC is
generously supplying the bandwidth.
We’ll also be showing brand new versions of FlowJo,
for both
Mac and Windows.
This will be our biggest release ever. If you’ve
heard
FlowJo is the flow cytometry software to beat, we’re about to raise
the
bar once again.
Au revoir,
Adam
————————————–…
Adam Treister
Tree Star, Inc.
340 A St.
Ashland OR 97520
1-800-366-6045
————————————–… Received on Wed May 12 16:38:00 2004
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03:12:03 EST
FlowJo hiring, come see us at ISAC
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From: Adam Treister a…@treestar.com
Date: Sun May 14 2006 - 20:04:57 EDT
Come join the FlowJo team!
We are looking to expand FlowJo’s market presence, train a
generation
of scientists in the subtle beauty of high color flow, and solve
some
small subset of the world’s problems.
If you’re coming to ISAC XXIII
in Quebec, and are interested in making the cytometry world a better
place,
please bring by your CV and talk to us in Booth 301.
If you don’t need a job,
at least come by to get a tie-dyed t-shirt.
If you’re not coming to ISAC,
you must not be cool,
and
we don’t want to hire you.
Unless, of course, you are an exceptional flow user,
with good grasp of the theory behind the machine,
know at least ten
ways to hold down modifier key
to perform silly FlowJo tricks,
and
want to share your expertise with a growing user community.
Then,
please send your CV to j…@treestar.com,
and
we’ll evaluate your feeble excuse
for missing this important conference.
We’ll send you a t-shirt if we have any left.
Adam
————————————-
Adam Treister
President
Tree Star, Inc.
340 A Street
Ashland OR 97520
www.flowjo.com
————————————-
Received on Mon May 15 14:18:00 2006
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This archive was generated by hypermail 2.1.8 : Tue May 16 2006 -
04:12:02 EDT
http://www.funbolt.com/articles/hiv-aids-25/purdue-cytometry-mail-list-isac-congress-67525/
Dear Robert Murphy
NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN
PROCESS 24,000-42,000 SAMPLES PER HOUR. FLOW CYTOMETRY FCS CYTO PRO
QUICK FACS http://www.kanecki.com/flow.html
Increased quality and productivity. With 10,000,000 event files, you
can process 24,000-samples/hour, and maintain quality up to Sigma 5
or
better. Compare this to having your research technologist performing only 100 samples/hour analysis.
Increased laboratory utilization by 3X because you can perform the
analysis off-lab and free laboratory time for reading samples.
This
was achieved when I developed the program, and we had a program
project grant from 1992 to 1998 of $8M.
Works with FCS 3.0 in all data modes as floating point, integer*4,
and
ASCII.
Works with BD and Beckman/Coulter Flow Cytometers and Cell Sorters
Backwards compatible with FCS 2.0 files and Flow Cytometers and Cell
Sorters.
Can read FCS 3.0 files up to 10M
events with 20 parameters.
Easy to
Use, three step process. Load initial file, set gate, specify file
list to process. That’s it.
Collaboration tools to allow you to cut and paste image results to
results.
Statistical analysis results imprinted on histogram plots directly as
mean, mode, and median with the ability to present results in log
mode
or linear mode, depending on the detector used. Plain vanilla coding
for easy update and maintenance to allow for the greatest user and
software quality.
One time purchase fee, no yearly renewal fees as with others.
Proven
tract record in FACS, Fluorescent Activated Cell Sorter Laboratory.
The laboratory was rated the best laboratory in the Midwest USA in
1990.
This application is designed for large-scale fluorescent activated
cell sorter
analysis. The program can read up to FCS 3.0 files and has been
tested
to run on
Becton Dickinson and CoulterOrtho based flow cytometers
and
cell sorters.
The main advantage of this program is that you can have
the computer perform the analysis for you after you have selected the
region to analyze. The result is that up to 24,000 samples per hour
can be analyzed on a 1.4 GHz speed computer. This program is designed
for researcher and technologist use. It uses rectangular gating, and
is intuitive to use. To use this program, the FCS must have the
extension, *.bin as 54203023.bin as an example. The *.bin extension
is what the computer uses to locate the files on the computer.
THANK YOU FOR YOU TIME MITCHELL HAYNES VP
SALES KANECKI
ASSOCIATES 832-347-1669
******************************************
NEVER MADE IT TO ISAC CONGRESS OR THE PURDUE CYTOMETRY MAIL LIST
Developed to resolve the BD Diva Memory ISSUES
*****************************************
****************************************
If you read the message from J Paul Robinson he stated we tried to Post on the list. Read the response from when he got the letter sent to Robert Murphy. I could had been Intercepted.
THIS WAS J PAUL ROBINSON RESPONSE TO THE INFORMATION SENT Re: Fw: NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN PROCESS 24,000 SAMPLES PER HOUR…Standard Header|Full Message
View J. Paul Robinson J. Paul Robinson … ViewFriday, September 28,
2007 9:37:32 AM To:mitchell haynes Cc:david_at_kanecki2.com;
skelley_at_flowcyt.cyto.purdue.edu
Steve
what is this email - it came to me with Bob Murphy’s name associated
with it. It seems to be an advertisement, this junk mail, and it
seems
to have been modified by you …
So I guess I am confused.
was this sent to the list,
or do you have
an
details about it
- i am concerned about these junk messages going out
to
our members,
if they are using our lists
I will deal with them
appropriately,
but I am not happy
any info you can give
thanks
paul
Response was sent to J. Paul Robinson
skelley_at_flowcyt.cyto.purdue.edu
Cc: skelley_at_flowcyt.cyto.purdue.edu
Sent: Friday, September 28, 2007 11:39:02 AM
Subject: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING INFORMATION TO PROPER
CHANNELS
Dear Paul,
I recieved your responce to the email I sent. Please understand it is not
junkmail but a update on new technology that will inhance all
flowcytometers..It is currently being evaluated by
BD who request for this
software to be developed directly by our corporation.
It was simply sent as an announcement for you concideration.
The software is demonstrates precision and a higer processing rate than every existing software today.
If you have any questions please call I provided my phone number with the email.
I understand institutions of your caliber is always looking for new
technology. Futhermoore this is the only software in the world that works
for every platform on one peice of software
Thank your for you time and have a great day.
Please do not blame Steve for send the information to the proper channels
I would think you would be upset if he did not foward important infomation
that pertains to furthering cytometry breakthroughs.
If you would like us to send information to another address that won’t
interfer please foward it to me and I will make sure that there are no
more misunderstandings.
Mitchell Haynes
From: jpr_at_flowcyt.cyto.purdue.edu
jpr_at_flowcyt.cyto.purdue.edu
To: mitchell haynes buybroker_at_yahoo.com
Cc: jpr_at_flowcyt.cyto.purdue.edu
Sent: Friday, September 28, 2007 2:26:51 PM
Subject: Re: Fw: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING
INFORMATION
TO
PROPER CHANNELS
Sorry, I think it is junk mail
regards
paul robinson
Advertising
From: Mario Roederer (roede…@drmr.com)
Date: Fri Nov 01 2002 - 16:46:47 EST
* Next message: William Telford: Re: Fluorochromes choice
* Previous message: Derek Davies: RE: Fluorochromes choice
* Next in thread: Penney Robbins: RE: Advertising
* Maybe reply: Penney Robbins: RE: Advertising
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[ author ]
[ attachment ]
________________________________________
I agree that there have been far too many commercial-oriented emails
on the list.
I appreciate the efforts of most manufacturers to
withhold from advertising
on this list
(plus, of course, the efforts
of Steve Paul to filter those out).
The list, which is an outstanding forum for exchange of information,
has been occasionally used to identify substantially
new products
which can significantly impact
on how
we do experiments.
I feel that
the Molecular Probes email of 10/30
clearly does not fall into this
category;
the new product advertised
was no more than a slight
modification of the existing one.
Such an email should be directed
solely to the person requesting information;
if that person then
collates responses and puts it back on the list then so be it.
But
for manufacturers to directly respond in this way is
counter-productive to the goals of this list.
I would like to propose a 6-month moratorium on all emails that are
no more than advertisements.
Note that I write would like
to–because
I’m not sure that this is possible.
I
don’t want to put
any additional onus on
Steve or Paul
to filter out the borderline
emails.
While these may be easy to identify when they come from
manufacturers,
it could just as well be considered
blatant advertising
when they come from a user.
Therefore,
perhaps
we
can see if the
commercial participants
on this
list could exercise self-restraint
rather than requesting a formal
censorship of advertising emails.
Thus, if you are a manufacturer, and you are responding to somebody’s
request for information,
do so privately to that person ONLY.
It is
up to the person requesting information to decide whether or not the
information received in response to the query warrants a summary on
the list.
If you are not a manufacturer,
and are responding to somebody’s
request for specific information,
please consider whether your
response
(that identifies a specific product or manufacturer)
is of
general enough interest to warrant the list.
If it does not, then
simply send it privately to the person who requested information,
and
let them decide whether to post the summary of responses.
In general, I urge people to err on the side of caution and send
their information only to the person who requested it.
Realize that
if several people want the same information,
they can always request
it from the original poster!
I have posted queries to the list;
people have sent me emails asking me to forward to them the
responses,
which I did.
This process can significantly cut down on emails
that might be
viewed as
too commercial.
mr
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:42:08 EST
Latest FlowJo focuses on presentation graphics
From: Adam Treister
(a…@treestar.com)
Date: Tue Dec 29 1998 - 10:34:17 EST
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________________________________________
There’s a new version of FlowJo now available on our web site.
Since
its
initial public release eighteen months ago,
FlowJo has had the
reputation
that it is very powerful in its batch processing,
but
didn’t have
enough
page layout capabilities to create publication quality reports
without
an
intermediate drawing program.
This release adds all those
capabilities.
We’ve added histogram overlays,
colored dot plots, more and more
flexible
statistics,
a palette of annotation tools, and a custom print preview
window to position as many graphs on a page as you wish,
without
fighting
the printer’s page breaks.
Or if you don’t want to print to paper,
we’ll
produce slide shows, animations or web pages directly from
FlowJo’s
layout
editor.
In addition, we’ve added a DNA / Cell Cycle platform to our Kinetics,
Compensation and Calibration tools. This new platform will fit
multiple
models to the histogram,
and let you tailor the models’ constraints
interactively.
Because its built into FlowJo,
you can easily combine
these
analyses with other phenotyping and batch analyses.
We encourage you to go take a look at:
http://www.treestar.com/flowjo
For the full list of features:
http://www.treestar.com/flowjo/html/version.html
Adam Treister
—————————–
Adam Treister
a…@treestar.com
http://www.treestar.com 800-366-6045
—————————–
Re: advertising
From: Alice L. Givan
(Alice.L.Gi…@dartmouth.edu)
Date: Wed Nov 06 2002 - 14:38:54 EST
* Next message: Amy Raber: degranulation-another question!
* Previous message: Robert Keefe: Y. pestis
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________________________________________
I need to add that
I was NOT suggesting that this network give any
special dispensation
to **** Haugland
and Molecular Probes.
They, like many other
companies,
are of
great benefit to the flow community.
I was suggesting that
we be a
bit relaxed in
our willingness to accept subtle advertising in general
— because
we can benefit
from being able to get advice directly
from people who
are at the
forefront of flow
development.
development.
Obviously,
knowing where to draw the line can be
difficult…..
Alice
Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
gi…@dartmouth.edu
________________________________________
* Next message: Amy Raber: degranulation-another question!
* Previous message: Robert Keefe: Y. pestis
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[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:42:08 EST
Re: advertising
From: Robb Habbersett
(r…@lanl.gov)
Date: Wed Nov 06 2002 - 14:19:13 EST
* Next message: Richard Haugland: Re: rat blood eosinophils
* Previous message: Amy Raber: degranulation-another question!
* Previous message: Robert Keefe: Y. pestis
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:42:08 EST
Re: advertising
From: Robb Habbersett (r…@lanl.gov)
Date: Wed Nov 06 2002 - 14:19:13 EST
* Next message: Richard Haugland: Re: rat blood eosinophils
* Previous message: Amy Raber: degranulation-another question!
* In reply to: Alice L. Givan: Re: advertising
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* Reply: Marty Bigos: Re: advertising
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[ attachment ]
________________________________________
Once again Alice is … RIGHT ON!!
Robb
At 05:52 PM 11/5/2002 -0500, Alice L. Givan wrote:
-
I would not like for **** Haugland
to feel that we are criticizing his
participation in
this list.
Molecular Probes
has been a HUGE help to all flow and imaging
cytometrists.
Advice from that company has been
good, generous, dependable,
and
without strings.
The new MP handbook is a fantastic source of information.
I would hope
that advice
from **** will continue to be submitted to the whole network —
and if he
gets a bit
of free advertising from it, then he deserves it.
Alice
Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
gi…@dartmouth.edu
Robert C. Habbersett
Technical Staff Member
Los Alamos National Laboratory
MS-M888 BN2
Los Alamos, NM 87545
phone 505.667.0296
fax 505.665.3024
As often happens in science, the paradox was resolved as soon as the
obvious was abandoned in the face of experimental evidence.
{from a recent review in Science}
________________________________________
* Next message: Richard Haugland: Re: rat blood eosinophils
* Previous message: Amy Raber: degranulation-another question!
* In reply to: Alice L. Givan: Re: advertising
* Next in thread: Marty Bigos: Re: advertising
* Reply: Marty Bigos: Re: advertising
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
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________________________________________
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17:42:09 EST
Re: simulator software
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From: Adam Treister a…@treestar.com
Date: Sun Mar 16 2008 - 00:04:42 EDT
Justin,
We’re working hard to finalize FlowJo Collectors’ Edition,
which
adds
Acquisition
(only for Cytek upgrades).
That version already has some
ability to simulate acquisition by reading an FCS file as the input,
but it ignores the machine settings when simulating so increasing
the
gain doesn’t show the increase in the data previews.
Making it work interactively in simulation mode has been postponed
while we smooth out how the program works when it’s actually
acquiring,
but we’ll get you a beta version to see if it’s a good
option for you.
Our intention
is to support simulation without
needing a license,
so it will be free for training purposes.
Check
out the web page: http://www.flowjo.com/v7/html/cypod.html
On a different note, I want to thank Allan Kachelmeier, et al for
putting on another great meeting in Portland this week. I took
several new employees up to Portland to expose them what we do, and
they were all blown away by what cool people are doing this
interesting science. Flowers rock.
Adam
Adam Treister
President
Tree Star, Inc.
J. Paul Robinson
jpr_at_flowcyt.cyto.purdue.edu
ViewMonday, November 19, 2007 2:14:38 PM
To:Larry Farrell farrlarr_at_isu.edu; mitchell haynes
buybroker_at_yahoo.com;
skelley_at_flowcyt.cyto.purdue.edu; Bartek Rajwa
rajwa_at_flowcyt.cyto.purdue.edu
Larry
Sorry you are experiencing a problem
From the header - This is apparently coming from a Mitchel Haynes
mitchell haynes buybroker_at_yahoo.com - is this correct?
I initially thought messages coming from this person were a scam and
challenged them when they started posting to our list as well. I had
someone do a check on them. it is apparent that they have written
some
software and they think its ants-pants. They have tried to post
things
on the Purdue list, they have used every possible website and email
discussion group to get cross listed to boost their ratings on
Google.
Basically
I view their behavior as very tenuous and from the message
you
sent me,
it appears that it is not appropriate to do what they are
doing…
I have taken the following action:
Steve Kelley
has been instructed to remove their email and any
postings on the Purdue list.
They are now banned
2. I am going to Copying Bartek Rajwa the editor of the ISAC site to
beware of them
3. I am contacting Google and other sites to let them know that these
people are self-propagating links.
4. If I see any message that in any way impunes Purdue or our
reputation,
I will go after them with every possible legal recourse
at
my disposal. We will not allow our reputation to suffer because of
commercial abuse.
5. You should ban this address, and basically indicate to your
members
that this is a scam.
While they claim to be legitimate, they are
acting
exactly as any scam artist…
I already told them that they were
acting
as scammers
and they got upset with me…well, if they don’t desist,
they will find out how much influence we actually have…
I will check the message
Subject: flow cytometry software will you trust Purdue..Lets play
MONOPOLY
Date: Mon, 19 Nov 2007 09:38:56 -0800 (PST)
Organization: http://groups.google.com
and see if this is actually libelous - it appears to be - and if it
is,
I will close this person down really, really fast!!! (Steve please
check
out this message and make a copy)
Larry,
we have about 3000 people
on the Purdue discussion list which
is
primarily about cytometry -
and a lot of flow cytometry - to join,
people actually have to go through me,
and we review every person who
comes on.
I am sorry you are experiencing this problem,
and we are happy to
help
in any way we can.
regards
paul Robinson
Professor, Purdue
GLarry Farrell wrote:
Someone has apparently decided to post the following, and a huge number
of related messages,
to misc.health.aids.
I have asked that person,
on
several occasions, not to post this material into that newsgroup and
have been ignored. My reasons for that request are two-fold: (1)
Misc.health.aids is an HIV/AIDS group so the material is decidedly off
topic,
and
(2) Some of these messages contain reference to a site from
which cytometry software
can be purchased and commercial solicitations
of any sort are expressly forbidden by the group’s charter.
Is there
any way you can intervene in this issue and stop the *many* posting
of
this and related cytometry messages in
misc.health.aids?
Dear Robert Murphy
NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN
PROCESS 24,000-42,000 SAMPLES PER HOUR. FLOW CYTOMETRY FCS CYTO PRO
QUICK FACS http://www.kanecki.com/flow.html
Increased quality and productivity. With 10,000,000 event files, you
can process 24,000 samples/hour, and maintain quality up to Sigma 5
or
better. Compare this to having your research technologist performing
only 100 samples/hour analysis.
Increased laboratory utilization by 3X because you can perform the
analysis off-lab and free laboratory time for reading samples.
This
was achieved when I developed the program, and we had a program
project grant from 1992 to 1998 of $8M.
Works with FCS 3.0 in all data modes as floating point, integer*4,
and
ASCII.
Works with BD and Beckman/Coulter Flow Cytometers and Cell Sorters
Backwards compatible with FCS 2.0 files and Flow Cytometers and Cell
Sorters.
Can read FCS 3.0 files up to 10M events with 20 parameters. Easy to
Use, three step process. Load initial file, set gate, specify file
list to process. That’s it.
Collaboration tools to allow you to cut and paste image results to
results.
Statistical analysis results imprinted on histogram plots directly as
mean, mode, and median with the ability to present results in log
mode
or linear mode, depending on the detector used. Plain vanilla coding
for easy update and maintenance to allow for the greatest user and
software quality.
One time purchase fee, no yearly renewal fees as with others. Proven
tract record in FACS, Fluorescent Activated Cell Sorter Laboratory.
The laboratory was rated the best laboratory in the Midwest USA in
1990.
This application is designed for large-scale fluorescent activated
cell sorter
analysis. The program can read up to FCS 3.0 files and has been
tested
to run on Becton Dickinson
and CoulterOrtho based flow cytometers and
cell sorters. The main advantage of this program is that you can have
the computer perform the analysis for you after you have selected the
region to analyze. The result is that up to 24,000 samples per hour
can be analyzed on a 1.4 GHz speed computer. This program is designed
for researcher and technologist use. It uses rectangular gating, and
is intuitive to use. To use this program, the FCS must have the
extension, *.bin as 54203023.bin as an example. The *.bin extension
is what the computer uses to locate the files on the computer.
THANK YOU FOR YOU TIME MITCHELL HAYNES VP
SALES KANECKI
ASSOCIATES 832-347-1669
Larry D. Farrell, Ph.D.
Professor of Microbiology
Idaho State University
Paul Robinson was *not* informed of my message to you. What makes
you
think he was?
I deleted the Undeliverable Mail notice as soon as it was received,
assuming that it was a dead issue since you would not have received the
message. And then, lo and behold, you actually did. Something is fishy!
Why do I care? Because you are flooding a normally useful newsgroup
with off-topic material, wasting bandwidth and turning off people who
might read the group. Your behavior in this group certainly does not
reflect well on you or on Kanecki Associates, the company for which you
apparently work as Marketing Director.
You have no idea how glad I am that you aren’t one of my students!!
—
Larry D. Farrell, Ph.D.
Professor of Microbiology
Idaho State University
Related messages: [ Next message ] [ Previous message ]
From: VSH - Tech Support t…@vsh.com
Date: Wed Jun 30 2004 - 12:08:14 EST
David
At the risk of sounding commercial, WinList can do what you want. I
have
attached a few pictures showing the WinList Region Array feature.
With this
you can tell the program exactly how you want the regions created and
how
many. If you would like to try it for yourself you can download a
trial
version from our web sitewww.vsh.com.
Please let me know if you have any questions.
Best regards
Don
Donald J. Herbert
Technical Support Manager
Verity Software House, Inc.
PO Box 247
45A Augusta Road
Topsham, ME, USA 04086
Phone: (207) 729-6767 ext.190
Fax: (207) 729-5443
email: t…@vsh.com
web:www.vsh.com
anti) Commercial Posting:)
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Ray Hicks rh…@cam.ac.uk
Date: Wed Oct 13 2004 - 08:43:13 EST
Just a quick nopte to let people know that FCSPress is now shareware
(no time restrictions or feature reductions for free use, though if
you feel like like paying for it you can using paypal).
It also now
works with Diva export files and runs ok under classic on OS 10.2 and
10.3. I’ve discontinued FCS Assistant, which has fallen behind
FCSPress remarkably, and which failed to run as shareware on some
systems
(it insisted you buy it
or refused to run -
FCSPress doesn’t
even have a nag screen now - pesky things).
FCSPress is available from
www.fcspress.com
Enjoy!
Ray
From: VSH Tech Support [mailto:T...@vsh.com]
Sent: Wednesday, February 21, 2007 10:30 AM
To: Cytometry Mailing List
Subject: RE: DNA analysis software
Hello Ibtissam,
ModFit LT, for PC or Mac, has advanced modeling capability for
research applications in DNA cell cycle analysis.
You may use any of
the model templates the program offers, or create your own models for
non-traditional analysis, including non-mammalian DNA cell cycle
studies.
ModFit LT can be linked to our WinList program
to provide a
complete cell cycle analysis on any number of sub-populations with a
single click of a button.
For an overview,
visit
http://www.vsh.com/products%a0.
Best regards,
Don
Donald J. Herbert
Technical Support Manager
Verity Software House
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN PROCESS 24,000 SAMPLES PER HOUR…
Standard Header|Full Message
View J. Paul Robinson J. Paul Robinson … ViewFriday, September 28,
2007 9:37:32 AM
To:mitchell haynes
Cc:david_at_kanecki2.com;
skelley_at_flowcyt.cyto.purdue.edu
Steve
what is this email -
it came to me with Bob Murphy’s name associated
with it.
It seems to be an advertisement,
this junk mail,
and it
seems
to have been modified by you …
So I guess I am confused.
was this sent to the list,
or do you have
an
details about it
- i am concerned
about these junk messages
going out
to
our members,
if they are using our lists
I will deal with them
appropriately,
but I am not happy
any info you can give
thanks
paul
FW: FlowJo Seminar for Los Angeles Basin
From: Barsky, Lora (LBar…@chla.usc.edu)
Date: Tue Mar 20 2001 - 14:28:04 EST
Next message: PLo…@adarc.org: Apoptosis, Annexin V and Ficoll
Previous message: Olson, Douglas: CCR7 Available
Next in thread: Sue DeMaggio: Re:
FW: FlowJo Seminar for Los Angeles
Basin
Reply: Sue DeMaggio: Re: FW: FlowJo Seminar for Los Angeles Basin
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
I’m not sure if there is a Los Angeles Basin users
group,
but thought
I could advertise here to interested parties.
Thanks,
Lora Barsky - Lead Operator
Research Immunology/BMT FACS Core
Childrens Hospital Los Angeles
Creating a database of FCS files
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ] [ Next in
thread ] [ Replies ]
From: VSH - Tech Support t…@vsh.com
Date: Tue Apr 27 2004 - 09:00:36 EST
Hello, Flow-ers,
With reference to databasing FCS file keywords, WinList from Verity
Software
has full databasing capability. The user may choose FCS file keywords
and
results keywords to database in a delimited ASCII text format. The
database
can be easily imported into Excel and similar programs. WinList
supports
batch processing, and is available for PC and Mac platforms. For more
information and to download a trial version, visit our web site at
www.vsh.com.
Mark Munson
Sales Manager, Verity Software House.
Received on Tue Apr 27 12:58:00 2004
This message: [ Message body ]
Next message: VSH - Tech Support: histogram raw data
Creating a database of FCS files
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ] [ Next in
thread ] [ Replies ]
From: VSH - Tech Support t…@vsh.com
Date: Tue Apr 27 2004 - 09:00:36 EST
Hello, Flow-ers,
With reference to databasing FCS file keywords, WinList from Verity
Software
has full databasing capability. The user may choose FCS file keywords
and
results keywords to database in a delimited ASCII text format. The
database
can be easily imported into Excel and similar programs. WinList
supports
batch processing, and is available for PC and Mac platforms. For more
information and to download a trial version, visit our web site at
www.vsh.com.
Mark Munson
Sales Manager, Verity Software House.
Received on Tue Apr 27 12:58:00 2004
This message: [ Message body ]
Next message: VSH - Tech Support: histogram raw data
Re: Happy Holidays and a New Year’s Proposal; Response Spam
From: J. Paul Robinson (j…@flowcyt.cyto.purdue.edu)
Date: Wed Dec 24 2003 - 16:39:47 EST
• Next message: J. Paul Robinson: High content Analysis meeting
• Previous message: Neal Benson: Re: Happy Holidays and a New Year’s
Proposal
• In reply to: Beverly Barton: Happy Holidays and a New Year’s
Proposal
• Next in thread: Gerstein, Rachel: FW: Happy Holidays and a New
Year’s Proposal
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
From J. Paul Robinson,
Purdue Server
I am responding to this quickly so as not to generate a vast number of emails on the subject.
The Purdue email list is a monitored list.
That means that we
monitor all the messages
…SPAM just can’t get through.
(Well I suppose it could, but it would be hard).
We do this to protect the quality of our list,
and to save you worry
that you will get trash.
It is time consuming and costly to maintain
this policy.
Monitoring is NOT done by 99% of other discussion groups.
They may use antispam software
(as we do as well)
but they don’t
monitor.
We have constantly requested
that people put quality subject headings
in messages.
Without proper subject headings, a message is in danger
of being instantly deleted
and so it should be probably be.
You should
know that the
to:
line in all messages from the Cytometry list
contains the message:
To: Cytometry Mailing List thus you can presort your emails to move
all these to the
PURDUE
mailbox or something similar.
This is a more universal solution to trying to force people to
manually enter a specific subject title -
which by the way, will
create
difficulties in sorting by subject if they are all the same.
Best wishes
J.PauL Robins
Purdue Cytometry Mailing List: Free software
- FCS Express
Although FCS Express can read files from all manufacturers,
and the lite version is only $99
(not quite free, but close :-),
it will only run on the PC. …
www.cyto.purdue.edu/hmarchiv/2003/2205.htm - 6k - Cached - Similar pages - Note this
More results from www.cyto.purdue.edu »
From: Joanne Lannigan [ mailto:jl...@cms.mail.virginia.edu]
Sent: Sun 3/16/2008 11:56 AM
To: cyto-inbox its
JSAN cell sorting and analysis system there
Please refrain from use of this list for commercial purposes.
The
ISAC website
has a place where you can post news releases about such
events or
information.
Thanks-
You will find over 700 posting that might help with the answers at this
Advenet FlowCytometry Site
http://advenet.com/flowcytometry/blog/default.aspx
Data Recovery
From: Steve Kelley (kelley@flowcyt.cyto.purdue.edu)
Date: Tue Sep 14 1993 - 04:26:41 EST
Next message: Ron Mickaels: 10BaseT Hookups for HP 3xx
Previous message: Dawson, Carolyn: WANT TO DISCUSS?
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
I got the following message with a question about whether it was
appropriate for the mailing list. As far as I’m concerned, anything
remotely connected to cytometry is fine, including advertisements,
as long as they don’t overwhelm the list. If anyone has different
opinions, we could discuss them, but in the meantime, I’ll continue
to distribute anything that comes in.
Steve
=-=-=-=-=-=-=-=-=-=-=
TO USERS OF BECTON DICKINSON CONSORT 30 32 WORKSTATIONS:
If you have experienced loss of data for what ever reason on
Bering or HP equipment (hard drive, diskette, Bernoulli cartridge
or tape) in the LIF or HFS format, and want that data recovered,
contact Richard Cox at Flow Cytometry Support for details.
************************************************************
* FLOW CYTOMETRY SUPPORT *
* PO Box 3450 *
* Saratoga, CA 95070-1450 *
* *
* Voice: 408.370.6327 *
* FAX: 408.370.6876 *
* email 2359766@mcimail.com *
*
——————————————————————————–
Next message: Ron Mickaels: 10BaseT Hookups for HP 3xx
Previous message: Dawson, Carolyn: WANT TO DISCUSS?
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:25:58 EST
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN PROCESS 24,000 SAMPLES PER HOUR…Standard Header|Full
Message
View J. Paul Robinson J. Paul Robinson ..
. ViewFriday, September 28,
2007 9:37:32 AM To:mitchell haynes
Cc:david_at_kanecki2.com;
skelley_at_flowcyt.cyto.purdue.edu
Steve
what is this email
- it came to me with Bob Murphy’s name associated
with it.
It seems to be an advertisement,
this junk mail, and it
seems
to have been modified by you …
So I guess I am confused. was this sent to the list, or do you have
an
details about it -
i am concerned about these junk messages going out
to
our members, -
if they are using our lists, I will deal with them
appropriately, but I am not happy about this - any info you can give
me
appreciated
thanks
Paul
2007 End of year message from Purdue
From: J. Paul Robinson
j…@flowcyt.cyto.purdue.edu
Date: Fri Dec 28 2007 - 13:43:46 EST
Beware, the end is nigh!
No, not an apocalyptic prediction - but 2007 is definitely coming to
an
end.
Not before time I would say - it s been a busy year.
But
I have
some strong words to end the year and
I am going to say them!!
Of
course
you don t have to read them!
Cytometry is now 40 years old
and
it s been sort of decaying a bit.
What
do I mean?
I am amazed at how
conservative
and
frankly boring
the field has become.
Why?
It s time to move to the 21st century folks.
I’m
getting older
and frankly,
its time to kick some ****
as my younger
colleagues often say
We talk so much like it is the same old
cytometry
it has always been.
Wake up people
- times are changing -
look at all
these new small companies
trying to stick their noses
in our field!
True we need to do the core work and do it well, but lets not forget
that fundamental tools of cell analysis are changing and if we don’t
keep ourselves up-to-date and educated on what’s happening….before
we
know it, a new field will emerge and we will be like the old electron
microscopists who are still wondering what happened ……
I know most of us work in the field and like what we do, but I think
its
time to open up a little and try to do some serious integration of
our
field. It s not happening very effectively on the most part I would
say.
Cytometry is about integration of the tools of the field into the
vast
reaches of biological problems that we can contribute to solving.
Cytometry is about advancement of the field, that means always
looking
ahead. ISAC will soon be the International Society for Advancement of
Cytometry a 21st century Society not a 20th century Society.
Cytometry is not flow cytometry!!
Let s not kid ourselves about this
folks. Cytometry is about measuring cells - however you do it - and
flow
cytometry is just one component of many. I understand that it may be
the
only tool some of you use - I don t want to take away from that or
de-emphasize its value or importance. But, we so often hear people
talk
about our field only in context of just flow cytometry. Recently,
when
we polled the ISAC community on changing our name from analytical
Cytology to Advancement of Cytometry we received comments like hey
I
don t do flow cytometry, so why are you reducing the breadth of the
field?
Ouch - they think cytometry means flow cytometry.
We have
a
long way to go before we convince the community that we cover all
aspects of cytometry. And let s also remember the growing membership
in
India and China (that s half the worlds population right there)
it
s
high time we paid much more attention to these countries as a field.
Awtar Krishan can t be the only person to drive cytometry training
and
education for 1.2 billion Indians can he? Well he has been up to now.
Who is taking on the mantle of training and education of cytometry in
China?
So here’s the scoop.
That’s one of the reasons why the
Purdue Web
portal
is going to change.
We tried to make the change this past year,
but
there were too many other things happening here to achieve it.
But
come
middle of 2008,
I am resolved that you will see a huge difference
in
the
Purdue site.
It s been the
default cytometry communication portal now
for
many, many years.
We have focused on
good clean fun with
cytometry
-
quality, timely, simple
- no spam.
Many people like that actually.
The
portal is almost overwhelming for us 22,000 daily page requests
with
over 2 Gigs daily download.
In 2007 alone,
downloads of 208,000
powerpoint files, 233,000 PDF files,
8800 movies,
38,000 word
document
The education pages and the Cytometry Discussion Archive are
the
most hit for sure.
Over 125,000 distinct files
from our portal were
accessed in 2007.
But all good things must come to an end.
Come July 2008,
the usual
Purdue web portal may well be no more.
It will be replaced with
something entirely new.
Hopefully most will find it more useful and
relevant -
some will not like it.
Maybe we will be able to make
everyone
happy
….ha!..C’est la vie.
Some of you will be beta testers
and
advisers I hope.
So my best wishes to all in the cytometry field for 2008.
Regarding
the
past year on the discussion list,
its been lively,……………..
To end 2007,
let me make a big plug for a program we began at the
2006
ISAC congress.
Gary Durack from iCyt
and myself
started a small
not-for-profit
charity called
Cytometry for Life
in response to
Stephen Lewis’
compelling plea for some low cost
CD4 devices.
Our
field
has done a lot of talking about this,
but
only a few people
have
really
tried to do anything practical.
Well, folks we have all been doing
cytometry for a very long time
- it’s time to do something.
Cytometry
for life
(http://www.cytometryforlife.org)
is working hard.
We have
made tremendous progress in just one year.
It would be great if
you
all
decided to jump on board
and play a
small part.
You can give money,
advice, moral support,
talk to your politicians,
community health-
care,
charities,
whatever.
But get involved
as be recognized
as the
cytometry
community
to solve this problem
of bringing low cost,
portable
devices
to the 65% or more of African’s who don t live in the cities and
towns
where current CD4 technologies are available.
Our goal is to work in
areas not being served by current technologies.
We have heard these
calls before,
but folks
we have to deal with this problem
- it’s your problem
if you call yourself
a cytometry person.
Email me if you
can
help -
consider donating to the program, let’s make it work. By the
end
of 2008, I want to be telling you that the program is getting to
people
who need this desperately.
Help us achieve that for 2008.
I hope many of you got hold of a copy of our new double DVD set
Cytometry 60 years of Innovation if not
ask your local rep from
virtually any company
in our field.
It might give you a good sense of
how strong the foundation in our field really is.
I will see many of
you
at the 2008 congress in Budapest. I know some of you think its going
to
be expensive so I took several hours myself and created a webpage for
the cheap ones out there
so
you have no excuses not to go…
(http://www.cyto.purdue.edu/flowcyt/cheapflights1.htm).
It’s been a privilege to serve for the past 19 months as
President of
ISAC.
I will gladly pass that hat to
Bob Murphy
in May.
ISAC is alive
and well -
membership is growing daily.
I would not be surprised to
see
us top 2000
by the end of the
Congress in May.
I know that about 60%
of
the members of this list
are NOT
ISAC members.
Perhaps you should
consider
joining the Society
that keeps many
of
you in business?
http://www.isac-net.org/
My best wishes for you all in 2008 from Purdue
Paul
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology Biomedical Engineering
Director, Purdue University Cytometry Laboratories
President, International Society for Analytical Cytology
Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: j…@flowcyt.cyto.purdue.edu
www.cyto.purdue.edu
Join ISAC - www.isac-net.org
Change lives today - www.cytometryforlife.org
Received on Fri Dec 28 15:18:00 2007
*
Data Files Wanted for Software Development Purdue Cytometry
Vantage/FCS 2.0 data files wanted
From: Eric Martz (emartz@MICROBIO.UMASS.EDU)
Date: Tue Jul 20 1993 - 13:19:13 EST
Next message: Steve Hilliard: User fee survey–ancient history!!
Previous message: Dave Coder: HP file recovery
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
Towards the goal of making my PC analysis program MFI compatible,
can
anyone out there provide me with some sample list mode data files from some
of the newer or rarer cytometers?
I’d particularly appreciate some files
from the FACS Vantage, or any files which begin FCS2.0.
It would be useful to have files with few and many parameters.
MFI can
currently handle up to 8 parameters. MFI cannot currently handle files
which use different resolutions for different parameters in the same file
(e.g. 256 channels for some and 1024 channels for others), so don’t send
that kind of file.
FTP (in !!BINARY!! mode) sample files (anonymous) to flowcyt.cyto.purdue.edu
in /pub/upload, and send me an email message so I’ll know what to look
for and who donated it.
To minimize redundancy, I already have examples of the following (MFI is
compatible with these):
BD FACScan/Star Research Software for HP 3.1 computers.
BD Consort 30 for HP 3.1 computers.
BD CellFit for HP 3.1 computers.
BD Lysys II versions 1.0, 1.1 for HP 3.2 computers.
BD FACS 440 with DOS 3 or VAX/VMS 4.7.
Coulter Elite w/ DOS 4, DOS 5.
Coulter EPICS MDADS/86 under DOS.
Coulter XL.
Verity Winlist 1.0 sample file.
(MFI 3.4c is available by anonymous BINARY ftp from flowcyt.cyto.purdue.edu
in /pub/martz. A beta test version of 3.4d with several new features and
some known bugs which will be fixed ‘real soon now’ is in
/pub/martz/beta.dir.)
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz emartz@microbio.umass.edu
Professor of Immunology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, University of Massachusetts, Amherst MA 01003
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
——————————————————————————–
Next message: Steve Hilliard: User fee survey–ancient history!!
Previous message: Dave Coder: HP file recovery
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
Purdue Cytometry Biosafty/software
RE: biosaftey/software
From: Eric Martz (emartz@microbio.umass.edu)
Date: Wed Sep 22 1993 - 14:01:23 EST
Next message: Doug Smoot: RE: biosaftey/software
Previous message: kharkins@iastate.edu: biosaftey/software
Maybe in reply to: kharkins@iastate.edu: biosaftey/software
Next in thread: Doug Smoot: RE: biosaftey/software
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
Regarding Kristi Harkins inquiry about ratioing 2 parameters in list
mode data, Peter Lopez (I believe) recently made a similar inquiry.
Bob Murphy replied that CALC4 (available on a.cfr.cmu.edu) can do
it on VAX/VMS. Both Keith Kelley and Doug Smoot pointed out that
Phoenix sells PC software which can ratio. My MFI program (available
on flowcyt.bio.umass.edu) cannot ratio.
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz emartz@microbio.umass.edu
Professor of Immunology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
——————————————————————————–
Next message: Doug Smoot: RE: biosaftey/software
Previous message: kharkins@iastate.edu: biosaftey/software
Maybe in reply to: kharkins@iastate.edu: biosaftey/software
Next in thread: Doug Smoot: RE: biosaftey/software
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:25:58 EST
Purdue Winlist Course only $400
WinList Course
From: Benjamin C. Hunsberger (75450.167@CompuServe.COM)
Date: Thu Sep 30 1993 - 14:42:55 EST
Next message: david w. galbraith: TIFF files
Previous message: cjgros@ccmail.monsanto.com: ISAC E-mail query
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
Verity Software still has a few openings for the fall WinList Course.
Anyone interested in attending should call or FAX as soon as possible (It
is expected to fill up quickly).
Information and a registration form can be FAXed to you upon request.
Tuition cost is $400.00 per person.
The class will have a maximum of 15
students with a lot of hands-on work.
FAX 207-729-5443
Phone 207-729-6767
——————————————————————————–
Next message: david w. galbraith: TIFF files
Previous message: cjgros@ccmail.monsanto.com: ISAC E-mail query
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
——————————————————————————–
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:25:58 EST
Re: EMAIL ABUSE - how to
stop
From: Adam Treister (a…@treestar.com)
Date: Mon Dec 16 2002 - 16:00:07 EST
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In reply to: J.Paul Robinson: EMAIL ABUSE - how to stop
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On Thursday, December 12, 2002, at 05:43 AM, J.Paul Robinson
wrote
Colleagues: I am sending out a copy of a message I have just sent to
RNWAY laboratories of South Korea and all 20 worldwide distributors
of
RNWAY products most of whom are highly reputable companies. I am only
sending it to you because I am going to propose to create a small
SCIENTISTS against EMAIL ABUSE type of revolutionary
action………
Paul,
Sounds like you’re advocating fighting disease by eradicating the
antigen
instead of boosting the immune response
You can organize
all
you want on eliminating the pest, but until they put a stamp tax on
email, a better approach is to let the
messages be out there, but have them filtered to oblivion before you ever see them.
In your case, the postmaster at Purdue is probably already filtering millions of messages a day that come to the thousands of email users on campus.
They are probably capable of shutting down any RNWAY
mail,
and
spreading the word to other postmasters
that
they also should filter
those messages.
So
if you can get the
IT people at the university
to tighten their
sieve,
that’s best.
Otherwise you have to switch to an email program
that has good
junk filters.
I think I get 500+ messages a day,
and only 10 to 20 make it past the junk filter.
Until last summer I was using Outlook Express and spam was a huge problem.
Since then I switched to the free Mail program in OS X,
which
just added special features for spam detection and removal. Its
probably 97% effective, and I haven’t found any false positives.
So, of course, the best answer is to get a Mac
I’m sure the PC mail clients are addressing this issue as well. I
believe there are central databases of offenders so programs can
learn
from others which messages to delete. I would imagine this is the
most important feature in any email program sold these days, so I bet
Eudora or other third party mail programs have this solved.
There’s a lot of information on the subject at:
http://spam.abuse.net/
Whether you fight the problem on the server or the client, it
definitely is worth getting it cleaned up. I found it screwed up my
whole communications process because every time I wanted check email,
I
had to wade through dozens or hundreds of useless ads.
Adam
—————————————————
Adam
Treister
***********************************
a…@treestar.com
www.flowjo.com 800-366-6045
From : J. Paul Robinson j…@flowcyt.cyto.purdue.edu
.We are
currently working on adding the support of these files in the Windows
version of FlowJo, …
www.cyto.purdue.edu/hmarchiv/2004/1020.htm
January is Flowjo month at URMC
Bushnell, Timothy [log in to unmask]
Wed, 2 Jan 2008 09:46:58 -0500
244 lines
Reminder: 1 pm - Flowjo Seminar
Bushnell, Timothy [log in to unmask]
Fri, 4 Jan 2008 10:12:35 -0500
193 lines
Reminder: Friday 1 pm - Flowjo Seminar on Compensation and
Transformation
Bushnell, Timothy [log in to unmask]
Wed, 9 Jan 2008 11:20:29 -0500
214 lines
Reminder: Friday 1 pm - Flowjo Seminar on DNA Analysis and Cell
Proliferation
Bushnell, Timothy [log in to unmask]
Wed, 16 Jan 2008 09:59:37 -0500
220 lines
Reminder: Today 1 pm - Flowjo Seminar on Compensation and
Transformation
Bushnell, Timothy [log in to unmask]
Fri, 11 Jan 2008 10:31:11 -0500
221 lines
Subject:Feedback requested on Flowjo web seminarsFrom:Bushnell,
Timothy [log in to unmask]Reply-To:Cytometry [log in to unmask],
Bushnell, TimothyDate:Wed, 23 Jan 2008 13:24:34 -0500Content-
Type:multipart/alternativeParts/Attachments:
Colleagues: For those of you who attended any of the Flowjo web
seminars the last three weeks, I would ask if you could send me your
feedback.
How did the web seminar format work for you? How was the instructor,
John Quinn? Were the selected topics appropriate? Would you like more
of these organized? If so, what topics would you like covered? What
could be done to improve the web seminars? Please send your comments
to me directly. Thanks in advance
Tim Timothy Bushnell, Ph.D.Research Assistant Professor, Pediatrics
and OncologyCo-Director, URMC Flow Cytometry FacilityOffice:
Larry,
we have about 3000 people on the Purdue discussion list
which
is
primarily about cytometry
- and a lot of flow cytometry
- to join,
people actually have to go through me,
and
we review every person who
comes on.
I am sorry you are experiencing this problem, and we are happy to
help
in any way we can.
regards
paul Robinson
Professor, Purdue
GLarry Farrell wrote:
Someone has apparently decided to post the following, and a huge number
of related messages, to misc.health.aids.
I have asked that person,
on
several occasions, not to post this material into that newsgroup
and
have been ignored.
My reasons for that request are two-fold:
(1)
Misc.health.aids is an HIV/AIDS group so the material is decidedly off
topic,
and
(2) Some of these messages contain reference to a site from
which cytometry software can be purchased and commercial solicitations
of any sort are expressly forbidden by the group’s charter.
Is there
any way
you can intervene in this issue and stop the *many* posting
of
this and related cytometry messages in misc.health.aids?
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN PROCESS 24,000 SAMPLES PER HOUR…Standard Header|Full Message
View J. Paul Robinson J. Paul Robinson … ViewFriday, September 28,
2007 9:37:32 AM To:mitchell haynes Cc:david_at_kanecki2.com;
skelley_at_flowcyt.cyto.purdue.edu
Steve
what is this email
- it came to me with
Bob Murphy’s name associated
with it.
It seems to be an advertisement,
this junk mail,
and it
seems
to have been modified by you …
So I guess I am confused.
was this sent to the list,
or do you have
an
details about it -
i am concerned about these junk messages going out
to
our members, -
if they are using our lists,
I will deal with them
appropriately,
but I am not happy about this
- any info you can give
me
appreciated
thanks
paul
Web Site Changes SPAM
Re: Web site changes
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From: Mario Roederer roederer@drmr.com
Date: Sat Sep 24 2005 - 15:05:50 EST
and undoubtedly saves me additional spam. I much prefer the
additional privacy.
mr
At 1:10 PM -0700 9/22/05, FloCyte Associates, INC wrote:
Please …. PLEASE may we have the old search capabilities for the
membership list back! We can not search the new site for names in
our areas, for compiling invitations to users groups, for searching
for colleagues if we only know their company or first names…. the
limitations of searching for members by their last name only makes
this feature of the website almost useless!
Thanks
Sue
Microtiter Plates FCM
J. Paul Robinson
(p…@flowcyt.cyto.purdue.edu)
Fri, 30 Oct 92 11:14:49 GMT
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Next message: ERIC MARTZ:
Free PC Software (MFI v. 3)
Previous message: Marty Bigos: Re: New? MESF Method
Could someone please comment
on how
well
CYTEC’s
suction
probe works
for
deliveringcells
from
96
well plates
to
the cytometer.
How does it mix
the
cells,
what is the cell loss,
any any other problems
that might be faced.
I
am interested in trying it
on
an Elite,
but
would like to hear
comments first.
Thanks
Paul Robinson
Purdue University Cytometry Labs
Next message: ERIC MARTZ:
Free PC Software (MFI v. 3)
Previous message:
Marty Bigos: Re: New? MESF Method
FCS compression
ERIC MARTZ (emartz@titan.ucc.umass.edu)
Fri, 23 Oct 92 14:38:01 EDT
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Next message: Steve Kelley: FCS compression
Previous message: ERIC MARTZ: Profile FCS?
I’d like a utility which removes unwanted information from FCS1.0 or FCS2.0 standard list mode files.
For FACScan files created by FACScan Research Software (ca. 1988), this could reduce the file size by about two-thirds!
What I have in mind is removing FL2 and FL3 for single
color work
(the FACScan software lacks this option although it is present in the FACStar software),
and
reducing the resolution from 1024 channels to 256 channels (which reduces the space consumed from 2 bytes per parameter-event to 1).
Such a utility might also offer an option of inserting additional sample description
or other information.
Does anyone know of such a utility?
I’m considering writing it
but
I’d rather not re-invent the wheel
(for the hundredth time or so).
–
/* - - - - - - - - - - - - - - - - - From - - - - - - - - - - - - - -
- - - -
Eric Martz emartz@titan.ucc.umass.edu Professor of
Immunology
Voice 413-545-2325, FAX 413-545-1578
Morrill IVN Rm 203, Dept Microbiol, Univ Mass, Amherst MA 01003
USA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
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Did Republicans vote against the bailout because of emails and calls from their constituents ?
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